2026 Price Guide,Myc

The biotin c-myc peptide is a highly valuable reagent in molecular biology research, particularly for the detection and purification of proteins tagged with the c-Myc epitope. This specialized peptide sequence, when conjugated with biotin, offers enhanced detection capabilities and plays a significant role in various experimental workflows. Understanding its properties, applications, and the underlying science is crucial for researchers aiming to effectively utilize this tool.

The c-Myc protein itself is a 62 kDa transcription factor encoded by the c-Myc gene on human chromosome 8q24. It is a fundamental regulator of cellular processes, including proliferation, differentiation, apoptosis, and cell cycle progression. Due to its critical roles, c-Myc is frequently implicated in various cancers, often exhibiting activated states in tumor cells. This makes the c-Myc protein a significant target for scientific investigation.

To facilitate the study of c-Myc and other proteins, researchers often employ epitope tags. The Myc-tag is a peptide tag derived from the human c-Myc protein, specifically recognized by antibodies like the well-known 9E10 monoclonal antibody. This tag is typically a short amino acid sequence, most commonly EQKLISEEDL, representing a specific portion of the c-Myc protein, often from its C-terminal region. This Myc-tag serves as a molecular handle, allowing for the specific capture and detection of recombinant proteins that have been engineered to include this tag.

The integration of biotin into the biotin c-myc peptide system significantly amplifies its utility. Biotin is a small vitamin that has an exceptionally high affinity for avidin and streptavidin. This strong interaction forms the basis of the biotin-streptavidin technology, a cornerstone of many sensitive detection assays. When a c-Myc-tagged protein is bound by an anti-c-Myc antibody that is conjugated to biotin, the biotin label can then be detected with high sensitivity by avidin or streptavidin conjugated to an enzyme (like HRP for ELISA or Western blotting) or a fluorescent marker. This greatly enhances signal amplification, allowing for the detection of even low-abundance proteins.

The biotin anti-c-myc Epitope Tag Antibody is a prime example of a reagent that leverages this principle. These antibodies are specifically designed to recognize the c-Myc epitope tag and are pre-conjugated with biotin. This ready-to-use format simplifies experimental setup and ensures consistent results. The 9E10 clone, which recognizes the EQKLISEEDL sequence, is particularly prevalent.

Beyond antibody detection, the biotin c-myc peptide itself can be used as a peptide for various applications. A c-Myc Peptide can be employed in immunoassays to displace c-Myc-tagged fusion proteins that have bound to anti-c-Myc antibodies. This technique is useful for validating antibody specificity or for certain purification strategies. These peptide sequences are often synthesized to correspond precisely to the epitope recognized by the antibody, such as the EQKLISEEDL sequence.

The applications of biotin c-myc peptide and related reagents are diverse. They are instrumental in:

* Western Blotting: Detecting the presence and relative amount of a Myc-tagged protein in a sample.

* Immunoprecipitation (IP): Isolating a Myc-tagged protein from a complex mixture for downstream analysis. This process often involves using an antibody that binds to the Myc-tag.

* ELISA (Enzyme-Linked Immunosorbent Assay): Quantifying the amount of a Myc-tagged protein.

* Immunofluorescence (IF) and Immunohistochemistry (IHC): Visualizing the localization of Myc-tagged proteins within cells or tissues.

* Flow Cytometry: Analyzing Myc-tagged proteins on a cellular level, often referred to as CyFlow™ c-Myc Biotin in some commercial offerings.

* Protein Purification: Facilitating the capture and purification of Myc-tagged proteins using affinity chromatography.

It is important to note that the Myc-tag is a peptide tag derived from the c-Myc protein, and while it shares sequence homology, it is distinct from the endogenous c-Myc protein itself. This distinction is crucial, as some reagents, like the Myc-Trap, are designed to avoid binding to endogenous c-Myc to specifically target the tagged protein.

In summary, the biotin c-myc peptide and its associated antibodies are indispensable tools for researchers studying protein expression, function, and interactions. The biotin conjugation, combined with the specificity of the Myc-tag, provides a powerful system for sensitive and reliable detection and purification of target proteins. The c-Myc protein's central role in cellular biology and its frequent dysregulation in disease further underscore the importance of these reagents in advancing scientific understanding and therapeutic development.